Abstract :Abstract
The primary objective of the research proposal is to study the wholesome effect of constituents isolated from Eisenia arborea as antidiabetic’s agents. In the first year of the proposal, extraction, fractionation of E. arborea was carried out with methanol, chloroform and ethyl acetate. These fractions and phloroglucinol isolated were found to possess a-glycosidase and a-amylase inhibition and cytotoxic effects experimentally. It was also proved by in silico studies that phloroglucinol bound to important catalytic residues of a-glycosidase and a-amylase enzymes. In the second year of the research, few other phlorotannin compounds (E1 and E2) were isolated and their role on glucose uptake in C2C12 cells was also studied. Studies are underway to characterize the compounds by NMR and LC-MS. Since in most of the instances seaweeds are consumed as a whole or as supplement, the activity is due to synergistic action of multiple components present in the extract. In order to support our hypothesis, network pharmacology approach through in silico methods was carried out to show the multiple activities of different phlorotannins present in the brown macroalgae.